Resources


Most of the resources below and much more are accessible at Mimubase, a centralized resource and information port for Mimulus research.

Genomes

 Mimulus lewisii LF10 Genome v1.1: This is the draft LF10 genome assembly generated in the study “Genetic dissection of a major anthocyanin QTL contributing to pollinator-mediated reproductive isolation between sister species of Mimulus” (Yuan et al. 2013. Genetics. 194: 255-263).

 Mimulus lewisii LF10 Genome v1.8: This is a much improved LF10 genome assembly generated in the Fall of 2015, containing ~5000 scaffolds with N50 of 180 Kb and was first used in the study “Molecular basis of overdominance at a flower color locus” (LaFountain et al., 2017. G3. 7: 3947-3954).

 Mimulus lewisii LF10 Genome v2.0: This is a chromosomal level assembly generated by 75x PacBio data and high-resolution intra-specific linkage map.

 Mimulus lewisii SL9 Genome v1.0: This is the draft genome assembly of the SL9 mapping line, generated in the study “Bulk segregant analysis of an induced floral mutant identifies a MIXTA-like R2R3 MYB controlling nectar guide formation in Mimulus lewisii” (Yuan et al. 2013. Genetics. 194: 523-528).

 Mimulus lewisii SL9 pseudoscaffolds: This is the pseudoscaffolds of SL9 generated by aligning the individual SL9 contigs to the M. guttatus chromosomes, used in the “Bulk segregant analysis of an induced floral mutant identifies a MIXTA-like R2R3 MYB controlling nectar guide formation in Mimulus lewisii” (Yuan et al. 2013. Genetics. 194: 523-528).

 Mimulus cardinalis CE10 Genome v1.1: This is the draft CE10 genome assembly generated in the study “Genetic dissection of a major anthocyanin QTL contributing to pollinator-mediated reproductive isolation between sister species of Mimulus” (Yuan et al. 2013. Genetics. 194: 255-263).

 Mimulus cardinalis CE10 Genome v2.0: This is a chromosomal level assembly generated by 60x PacBio data and high-resolution intra-specific linkage map.

 Mimulus verbenaceus MvBL Genome v1.1: This is the MvBL genome assembly generated using 60x Pac-Bio data, with a contig N50 of 1.1 Mb.

 Mimulus parishii Mpar Genome v1.1: This is the Mpar genome assembly generated using 60x Pac-Bio data, with a contig N50 of 2.0 Mb.


Transcriptomes

 Mimulus lewisii LF10 Transcriptome v1.1: This is the LF10 15-mm corolla transcriptome generated in the study “Transcriptonal control of florla anthocyanin pigmentation in monkeyflowers (Mimulus)” (Yuan et al. 2014. New Phytologist. 204: 1013–1027).

 Mimulus lewisii LF10 Transcriptome v1.2: This is an updated transcriptome generated from LF10 leaf tissue and several flower developmental stages (including the calyx, corolla, stamens, and pistil).

 Mimulus verbenaceus MvBL Transcriptome v1.0: This is a transcriptome generated from MvBL leaf tissue and several flower developmental stages (including the calyx, corolla, stamens, and pistil).


TE libraries

 Mimulus guttatus TE exemplar library.


Protocols

 Mimulus lewisii in planta transformation

 RNAi constructs

 Over-expression and rescue constructs